http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/257 Sun, 05 Apr 2026 19:32:27 GMT 2026-04-05T19:32:27Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1611 Title: Formulation of double emulsions of mango seed extract (Mangifera indica L.) 'Ataulfo' incorporated into a mango by-product flour drink: Release kinetics, antioxidant capacity, and inhibition of cyclooxygenases Authors: Martínez Olivo, Abraham Osiris; Zamora Gasga, Víctor Manuel; Medina Torres, Luis; Pérez Larios, Alejandro; Sánchez Burgos, Jorge Alberto Abstract: Abstract: The encapsulation and release of bioactive compounds obtained from by-products are aspects of exponential boom for several decades, as it seeks to maintain or enhance their activity. A double emulsion (W1/O/W2) was developed with mango seed extract (MS) 'Ataulfo', said extract contains gallic acid and pentagalloyl glucose as major compounds (80.16%). The double emulsion was subjected to release kinetics for 3 h in phosphate buffer (pH 6.9), presenting a release constant (k) of 35,350 ± 6,031 μg/mL/min, in addition to antioxidant capacity by the DPPH and FRAP method of 168,663 ± 4,273 and 39,718 ± 1,019 mMol/g of double emulsion respectively at 120 min of kinetics, the time of 120 min was determined as the latency time (l). The release behavior corresponds to zero-order kinetics since the release of the extract remains constant until the minimum concentration is reached to exert the antioxidant capacity mentioned above. The mechanism of release of the SM extract contained in the double emulsion is governed by diffusion (Fickian behavior), this was determined thanks to the equations of the Korsmeyer-Peppas mathematical model, obtaining a regression adjustment (R2) of 0.9252 for said model and R2 of 0.8126 for zero-order kinetics. The double emulsion was added to a mango peel drink formulation, to which the antitopoisomerase activity was determined in strains of S. cerevisiae (JN394 and JN362a), however, no inhibitory activity was presented towards any strain. The cyclooxygenase inhibition (COX) assay was performed on the 120-minute released fraction and the MS extract, showing that this fraction only showed 18.97% inhibition in COX-II, however, the SM extract obtained an inhibition percentage of 38.14% in COX-II. Description: Artículo Fri, 01 Dec 2023 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1611 2023-12-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1588 Title: Identification, Viability, and Membrane Potential during the Cryopreservation of Autochthonous Lactic-Acid Bacteria Isolated from Artisanal Adobera Cheese from Los Altos de Jalisco Authors: Arteaga Garibay, Ramón Ignacio; Delgado Macuil, Raúl Jacobo; Gómez Godínez, Lorena Jacqueline; Cruz Cárdenas, Carlos Iván; Villagrán de la Mora, Blanca Zuamí; Giono Cerezo, Silvia; Zelaya Molina, Lily Xochitl; Anaya Esparza, Luis Miguel; Ruvalcaba Gómez, José Martín Abstract: Abstract Lactic acid bacteria (LAB) comprise a group of microorganisms responsible for developing the sensory and chemical characteristics of several foods and fermented products, particularly cheese. For this reason, after isolation and identification of LAB, validated protocols and procedures for their long-term preservation without compromising its integrity and technological properties, as well as methodologies aiming to assess their viability and integrity are paramount. This study aimed to isolate and identify autochthonous LAB from artisanal Adobera cheese and determine the effect of LAB cryopreservation with thioglycolate broth and glycerol on their viability, membrane integrity, and kinetics. Sixteen LAB were isolated and genetically identified from artisanal cheese samples; eleven of those strains were selected (genus Lactobacillus, Leuconostoc, Streptococcus, and Lactococcus) and included in the cryo-preservation assay. The initial average concentration of the bacterial suspensions was 6.89 log10 CFU mL−1; increasing to 8.9 log10 CFU mL−1 21 days later and slightly reduced at day 42 post-preservation (losses below one logarithm). About 77% of the cells maintained their membrane potential 180 days after their preservation and showed normal Kinetic parameters, maintaining normal adaptation times (Lag phase) and Log phases (9 h average), before reaching the stationary phase. The proposed protocol constitutes a viable alternative to the long-term preservation of different LAB genera because it keeps their viability and integrity. Using flow cytometry allowed the enumeration of viable LAB and provide evidence of the integrity of their membrane. Description: Artículo Fri, 01 Dec 2023 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1588 2023-12-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1587 Title: Design of Experiments for Optimizing Ultrasound-Assisted Extraction of Bioactive Compounds from Plant-Based Sources Authors: Anaya Esparza, Luis Miguel; Aurora Vigo, Edward F.; Villagrán de la Mora, Blanca Zuamí; Rodríguez Lafitte, Ernesto; Ruvalcaba Gómez, José Martín; Solano Cornejo, Miguel Ángel; Zamora Gasga, Victor Manuel; Montalvo González, Efigenia; Gómez Rodríguez, Horacio; Aceves Aldrete, César Eduardo; González Silva, Napoleón Abstract: Plant-based materials are an important source of bioactive compounds (BC) with interesting industrial applications. Therefore, adequate experimental strategies for maximizing their recovery yield are required. Among all procedures for extracting BC (maceration, Soxhlet, hydro-distillation, pulsed-electric field, enzyme, microwave, high hydrostatic pressure, and supercritical fluids), the ultrasound-assisted extraction (UAE) highlighted as an advanced, cost-efficient, eco-friendly, and sustainable alternative for recovering BC (polyphenols, flavonoids, anthocyanins, and carotenoids) from plant sources with higher yields. However, the UAE efficiency is influenced by several factors, including operational variables and extraction process (frequency, amplitude, ultrasonic power, pulse cycle, type of solvent, extraction time, solvent-to-solid ratio, pH, particle size, and temperature) that exert an impact on the molecular structures of targeted molecules, leading to variations in their biological properties. In this context, a diverse design of experiments (DOEs), including full or fractional factorial, Plackett–Burman, Box-Behnken, Central composite, Taguchi, Mixture, D-optimal, and Doehlert have been investigated alone and in combination to optimize the UAE of BC from plant-based materials, using the response surface methodology and mathematical models in a simple or multi-factorial/multi-response approach. The present review summarizes the advantages and limitations of the most common DOEs investigated to optimize the UAE of bioactive compounds from plant-based materials. Description: Artículo Fri, 01 Dec 2023 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1587 2023-12-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1580 Title: Growth Performance and Fecal Microbiota of Dairy Calves Supplemented with Autochthonous Lactic Acid Bacteria as Probiotics in Mexican Western Family Dairy Farming Authors: Ruvalcaba Gómez, José Martín; Villaseñor González, Fernando; Espinosa Martínez, Mario Alfredo; Gómez Godínez, Lorena Jacqueline; Rojas Anaya, Edith; Villagrán de la Mora, Blanca Zuamí; Anaya Esparza, Luis Miguel; Buendía Rodríguez, Germán; Arteaga Garibay, Ramón Ignacio Abstract: Probiotic supplementation in dairy cattle has achieved several beneficial effects (improved growth rate, immune response, and adequate ruminal microbiota). This study assessed the effects on the growth parameters and gut microbiota of newborn dairy calves supplemented with two Lactobacillus-based probiotics, individually (6BZ or 6BY) or their combination (6BZ + 6BY), administrated with the same concentration (1 × 109 CFU/kg weight) at three times, between days 5 and 19 after birth. The control group consisted of probiotic-unsupplemented calves. Growth parameters were recorded weekly until eight weeks and at the calves’ ages of three, four, and five months. Fecal microbiota was described by high-throughput sequencing and bioinformatics. Although no significant effects were observed regarding daily weight and height gain among probiotic-supplemented and non-supplemented calves, correlation analysis showed that growth rate was maintained until month 5 through probiotic supplementation, mainly when the two-strain probiotics were supplied. Modulation effects on microbiota were observed in probiotic-supplemented calves, improving the Bacteroidota: Firmicutes and the Proteobacteria ratios. Functional prediction by PICRUSt also showed an increment in several pathways when the two-strain probiotic was supplemented. Therefore, using the three-administration scheme, the two-strain probiotic improved the growth rate and gut microbiota profile in newborn dairy calves. However, positive effects could be reached by applying more administrations of the probiotic during the first 20 days of a calf’s life. Description: Artículo Fri, 01 Sep 2023 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1580 2023-09-01T00:00:00Z