Tumour necrosis factor-alpha polymorphism -308 G/a and its protein in subjects with gingivitis

Abstract

AbstractObjective This study examined the association between tumour necrosis factor-alpha (TNF- α) (?308?G/A) polymorphism and gingivitis, and serum and salivary TNF- α levels, in a Mexican population.Material and methods This study enrolled 171 subjects, divided into two groups: healthy subjects and gingivitis patients. TNF- α (?308?G/A) gene polymorphism was analyzed by PCR-RFLP assay. Salivary and serum samples were used to measure cytokine levels through the ELISA technique.Results TNF- α (?308?G/A) polymorphism was shown to have a protective effect in carriers of the A/A genotype and allele A. The G/A genotype is associated with an increase in high-density lipoprotein cholesterol (HDL-C) levels in the gingivitis group. Healthy individuals had higher levels of salivary TNF- α and HDL-C, and increased salivary flow. Triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol levels were increased in the gingivitis group. No statistical differences were found in serum TNF- α levels.Conclusion Our data demonstrate that the TNF- α ?308?A/A genotype exerts a protective effect against gingivitis. Moreover, oral conditions are associated with some biochemical parameters.