Please use this identifier to cite or link to this item: http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1343
Title: In vivo induction of neutrophil extracellular traps by Mycobacterium tuberculosis in a guinea pig model
Authors: Filio Rodríguez, Georgina
Estrada Gracía, Iris
Arce Paredes, Patricia
Moreno Altamirano, María Maximina Bertha
Islas Trujillo, Sergio
Ponce Regalado, María Dolores
Rojas Espinosa, Oscar
Keywords: in vivo
neutrophils
NETs
mycobacterium tuberculosis
guinea pig
Issue Date: Sep-2017
Publisher: PubMed
Citation: Filio-Rodríguez G, Estrada-García I, Arce-Paredes P, et al. In vivo induction of neutrophil extracellular traps by Mycobacterium tuberculosis in a guinea pig model. Innate Immunity. 2017;23(7):625-637. doi:10.1177/1753425917732406
Series/Report no.: Innate Immunity;Vol 23, Issue 7, 2017
Abstract: Abstract In 2004, a novel mechanism of cellular death, called ‘NETosis’, was described in neutrophils. This mechanism, different from necrosis and apoptosis, is characterized by the release of chromatin webs admixed with microbicidal granular proteins and peptides (NETs). NETs trap and kill a variety of microorganisms. Diverse microorganisms, including Mycobacterium tuberculosis, are NET inducers in vitro. The aim of this study was to examine whether M. tuberculosis can also induce NETs in vivo and if the NETs are bactericidal to the microorganism. Guinea pigs were intradermally inoculated with M. tuberculosis H37Rv, and the production of NETs was investigated at several time points thereafter. NETs were detected as early as 30 min post-inoculation and were clearly evident by 4 h post-inoculation. NETs produced in vivo contained DNA, myeloperoxidase, elastase, histones, ROS and acid-fast bacilli. Viable and heat-killed M. tuberculosis, as well as Mycobacterium bovis BCG were efficient NET inducers, as were unilamellar liposomes prepared with lipids from M. tuberculosis. In vitro, guinea pig neutrophils also produced NETs in response to M. tuberculosis. However, neither the in vivo nor the in vitro-produced NETs were able to kill M. tuberculosis. Nevertheless, in vivo, neutrophils might propitiate recruitment and activation of more efficient microbicidal cells.
Description: Artículo
URI: http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1343
ISSN: 17534259 print
17534267 eprint
Appears in Collections:3212 Artículos



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