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Title: | The 50kDa metalloproteinase TvMP50 is a zinc-mediated Trichomonas vaginalis virulence factor |
Authors: | Puente Rivera, Jonathan Villaplando, José Luis Villalobos Osnaya, Alma Vázquez Carrillo, Laura Isabel León Ávila, Gloria Ponce Regalado, María Dolores López Camarillo, César Elizalde Contreras, José Miguel Ruiz May, Eliel Arroyo, Rossana Arroyo Álvarez Sánchez, María Elizbeth |
Keywords: | trichomonas vaginalis Zn2+ TvMP50 cytotoxicity DU145 cells virulence factor |
Issue Date: | Oct-2017 |
Publisher: | Elsevier - Science Direct |
Citation: | Jonathan Puente-Rivera, José Luis Villalpando, Alma Villalobos-Osnaya, Laura Isabel Vázquez-Carrillo, Gloria León-Ávila, María Dolores Ponce-Regalado, César López-Camarillo, Jose Miguel Elizalde-Contreras, Eliel Ruiz-May, Rossana Arroyo, María Elizbeth Alvarez-Sánchez, The 50kDa metalloproteinase TvMP50 is a zinc-mediated Trichomonas vaginalis virulence factor, Molecular and Biochemical Parasitology, Volume 217, 2017, Pages 32-41, ISSN 0166-6851, https://doi.org/10.1016/j.molbiopara.2017.09.001. |
Series/Report no.: | Molecular and Biochemical Parasitology;Volume 217, October 2017, Pages 32-41 |
Abstract: | Trichomonas vaginalis is a protozoan parasite that can adapt to the trichomonicidal Zn2+ concentrations of the male urogenital tract microenvironment. This adaptation is mediated by molecular mechanisms, including proteinase expression, that are regulated by cations such as Zn2+. Herein, we characterized the previously identified 50kDa metalloproteinase aminopeptidase P (M24 family) member TvMP50 as a new Zn2+-mediated parasite virulence factor. Quantitative RT-PCR and indirect immunofluorescence assays corroborated the positive regulation of both mp50 gene expression and native TvMP50 protein overexpression in the cytoplasm and secretion products of parasites grown in the presence of Zn2+. Furthermore, this active metalloproteinase was characterized as a new virulence factor by assaying cytotoxicity toward prostatic DU145 cell monolayers as well as the inhibition of parasite and secreted soluble protein proteolytic activity in the 50kDa proteolytic region by the specific metalloproteinase inhibitor 1,10-phenanthroline and the chelating agents EDTA and EGTA. Parasite and secreted soluble protein cytotoxicity toward DU145 cells were reduced by treatment with an α-rTvMP50 polyclonal antibody. Our results show that the metalloproteinase TvMP50 is a new virulence factor modulated by Zn2+, which is present during male trichomoniasis, possibly explaining T. vaginalis survival even within the adverse conditions of the male urogenital microenvironment. |
Description: | Artículo |
URI: | http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1346 |
ISSN: | 0166-6851 |
Appears in Collections: | 3212 Artículos |
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File | Description | Size | Format | |
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The 50 kDa metalloproteinase TvMP50.pdf | Documento | 2.07 MB | Adobe PDF | View/Open |
Enlace a_The 50 kDa metalloproteinase TvMP50.htm | Enlace a publicación | 48.33 kB | HTML | View/Open |
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