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Title: | Recombinant Trichomonas vaginalis eIF-5A protein expressed from a eukaryotic system binds specifically to mammalian and putative trichomonal eIF-5A response elements (EREs) |
Authors: | Carvajal Gamez, Bertha Isabel Vázquez Carrillo, Laura Torres Romero, Julio César Camacho Nuez, Minerva Ponce Regalado, María Dolores López Camarillo, César Álvarez Sánchez, María Elizbeth |
Keywords: | trichomonas vaginalis eukaryotic translation initiation factor 5A TveIF-5A polyamines eIF-5A response elements EREs |
Issue Date: | Dec-2016 |
Publisher: | Elsevier - Science Direct |
Citation: | Carvajal-Gamez BI, Carrillo LV, Torres-Romero JC, Camacho-Nuez M, Ponce-Regalado MD, Camarillo CL, Alvarez-Sánchez ME. Recombinant Trichomonas vaginalis eIF-5A protein expressed from a eukaryotic system binds specifically to mammalian and putative trichomonal eIF-5A response elements (EREs). Parasitol Int. 2016 Dec;65(6 Pt A):625-631. doi: 10.1016/j.parint.2016.09.004. Epub 2016 Sep 9. PMID: 27620329. |
Series/Report no.: | Parasitology International;Volume 65, Issue 6, Part A, December 2016, Pages 625-631 |
Abstract: | Abstract Trichomonas vaginalis eIF-5A-like protein (TveIF-5A) belongs to the highly conserved eIF-5A family of proteins that contains a unique polyamine-derived amino acid, hypusine. Recently, we determined that the polyamine putrescine is required for tveif-5a mRNA stability, and it is necessary for stability and maturation of the TveIF-5A protein. Eukaryotic eIF-5A is known to be involved in mRNA turnover and is capable of sequence-specific RNA binding to eIF-5A response elements (EREs). These ERE sequences are present in diverse mammalian mRNAs, including human cyclooxygenase-2 ( cox-2 ). Here, we cloned the complete coding sequence of TveIF-5A and overexpressed it in a eukaryotic system. The recombinant protein (rTveIF-5A) was purified in soluble form using size-exclusion chromatography. Because of the polyamine-dependent regulation of TvCP39 (a protease of T. vaginalis ) at the protein and RNA messenger (mRNA) levels, we looked for an ERE-like structure in the 3′ region of tvcp39 mRNA. In RNA gel-shift assays, rTveIF-5A bound to transcripts at the EREs of cox-2 or tvcp39 mRNAs. This work shows the eIF-5A/ERE-like interaction in T. vaginalis . |
Description: | Artículo |
URI: | http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1347 |
ISSN: | 1383-5769 |
Appears in Collections: | 3212 Artículos |
Files in This Item:
File | Description | Size | Format | |
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Recombinant Trichomonas vaginalis.pdf | Documento | 748.31 kB | Adobe PDF | View/Open |
Enlace a_Recombinant Trichomonas vaginalis.htm | Enlace a publicación | 49.57 kB | HTML | View/Open |
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