Implications of the 5-lipoxygenase allelic variants c.760G>A and -1279 G>T in the nutritional and inflammatory status in Mexican Young Population

Abstract

Background: The arachidonate 5-lipoxygenase enzyme (5-LOX), which is encoded by the ALOX5 gene, plays a key role in mediating inflammation. Actually, 5-LOX represents a novel target for prevention of metabolic syndrome. Whether ALOX5 genetic variants associate with 5-LOX activity and pathophysiological events is unclear, and interactions of the allelic variants c.760G>A and -1279 G>T with nutritional and inflammatory status have not been studied in Mexican young population. Objective: The present research examines the ALOX5 gene c.760G>A and -1279 G>T variants and their cross-sectional association with inflammatory and nutritional status. Methods: Young volunteers students between the ages of 18 to 25 years old were recruited in Los Altos University Center. Institutional approval was obtained and all participants gave informed consent. Anthropometrical, biochemical, clinical and dietetic evaluations were performed. Genotyping was realized by Taqman Real-Time PCR Assays. The inflammatory biomarkers IL-6, TNFa and CRP were assayed by ELISA. Results: A total of 549 participants (26% men; 74% women) were studied. The frequency of c.760 G>A showed a significant difference between subjects with high vs normal serum levels of very low density lipoprotein cholesterol (c-VLDL) (p=0.007); moreover, almost significant difference was observed between subjects with high vs normal serum levels of c-LDL (p=0.07). Regarding inflammatory cytokines, a statistical trend was found on the IL-6 serum levels between c.760 G>A genotypes. No significant associations between -1279 G>T genotypes and inflammatory markers were observed. Conclusion: Our findings suggest that the c.760 G>A in the ALOX5 gene could be associated with inflammation and nutritional status.