Please use this identifier to cite or link to this item: http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1613
Title: Long chain capsaicin analogues synthetized by CALB‑CLEAs show cytotoxicity on glioblastoma cell lines
Authors: Diaz Vidal, Tania
Armenta Pérez, Vicente Paúl
Rosales Rivera, Luis Carlos
Basulto Padilla, Georgina Cristina
Martínez Pérez, Raúl Balam
Mateos Díaz, Juan Carlos
Gutiérrez Mercado, Yanet Karina
Canales Aguirre, Alejandro A.
Rodríguez, Jorge A.
Keywords: cross-linked enzyme aggregates
capsaicin analogues
candida antarctica lipase B
punivanil
punicic acid
glioblastoma
Issue Date: Dec-2024
Publisher: Springer Nature
Citation: Diaz-Vidal, T., Armenta-Pérez, V.P., Rosales-Rivera, L.C. et al. Long chain capsaicin analogues synthetized by CALB-CLEAs show cytotoxicity on glioblastoma cell lines. Appl Microbiol Biotechnol 108, 106 (2024). https://doi.org/10.1007/s00253-023-12856-y
Series/Report no.: Applied Microbiology and Biotechnology;Vol. 108, Issue 1. Article: 106. 2024
Abstract: Abstract Glioblastoma is one of the most lethal tumors, displaying striking cellular heterogeneity and drug resistance. The prognosis of patients suffering from glioblastoma after 5 years is only 5%. In the present work, capsaicin analogues bearing modifications on the acyl chain with long-chain fatty acids showed promising anti-tumoral activity by its cytotoxicity on U-87 and U-138 glioblastoma multiforme cells. The capsaicin analogues were enzymatically synthetized with cross-linked enzyme aggregates of lipase B from Candida antarctica (CALB). The catalytic performance of recombinant CALB-CLEAs was compared to their immobilized form on a hydrophobic support. After 72 h of reaction, the synthesis of capsaicin analogues from linoleic acid, docosahexaenoic acid, and punicic acid achieved a maximum conversion of 69.7, 8.3 and 30.3% with CALB-CLEAs, respectively. Similar values were obtained with commercial CALB, with conversion yields of 58.3, 24.2 and 22% for capsaicin analogues from linoleic acid, DHA and punicic acid, respectively. Olvanil and dohevanil had a significant cytotoxic effect on both U-87 and U-138 glioblastoma cells. Irrespective of the immobilization form, CALB is an efficient biocatalyst for the synthesis of anti-tumoral capsaicin derivatives. biocatalyst for the synthesis of anti-tumoral capsaicin derivatives.
Description: Artículo
URI: http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1613
ISSN: 1432-0614 online
0175-7598 print
https://doi.org/10.1007/s00253-023-12856-y
Appears in Collections:2414 Artículos

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