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Title: | Long chain capsaicin analogues synthetized by CALB‑CLEAs show cytotoxicity on glioblastoma cell lines |
Authors: | Diaz Vidal, Tania Armenta Pérez, Vicente Paúl Rosales Rivera, Luis Carlos Basulto Padilla, Georgina Cristina Martínez Pérez, Raúl Balam Mateos Díaz, Juan Carlos Gutiérrez Mercado, Yanet Karina Canales Aguirre, Alejandro A. Rodríguez, Jorge A. |
Keywords: | cross-linked enzyme aggregates capsaicin analogues candida antarctica lipase B punivanil punicic acid glioblastoma |
Issue Date: | Dec-2024 |
Publisher: | Springer Nature |
Citation: | Diaz-Vidal, T., Armenta-Pérez, V.P., Rosales-Rivera, L.C. et al. Long chain capsaicin analogues synthetized by CALB-CLEAs show cytotoxicity on glioblastoma cell lines. Appl Microbiol Biotechnol 108, 106 (2024). https://doi.org/10.1007/s00253-023-12856-y |
Series/Report no.: | Applied Microbiology and Biotechnology;Vol. 108, Issue 1. Article: 106. 2024 |
Abstract: | Abstract Glioblastoma is one of the most lethal tumors, displaying striking cellular heterogeneity and drug resistance. The prognosis of patients suffering from glioblastoma after 5 years is only 5%. In the present work, capsaicin analogues bearing modifications on the acyl chain with long-chain fatty acids showed promising anti-tumoral activity by its cytotoxicity on U-87 and U-138 glioblastoma multiforme cells. The capsaicin analogues were enzymatically synthetized with cross-linked enzyme aggregates of lipase B from Candida antarctica (CALB). The catalytic performance of recombinant CALB-CLEAs was compared to their immobilized form on a hydrophobic support. After 72 h of reaction, the synthesis of capsaicin analogues from linoleic acid, docosahexaenoic acid, and punicic acid achieved a maximum conversion of 69.7, 8.3 and 30.3% with CALB-CLEAs, respectively. Similar values were obtained with commercial CALB, with conversion yields of 58.3, 24.2 and 22% for capsaicin analogues from linoleic acid, DHA and punicic acid, respectively. Olvanil and dohevanil had a significant cytotoxic effect on both U-87 and U-138 glioblastoma cells. Irrespective of the immobilization form, CALB is an efficient biocatalyst for the synthesis of anti-tumoral capsaicin derivatives. biocatalyst for the synthesis of anti-tumoral capsaicin derivatives. |
Description: | Artículo |
URI: | http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1613 |
ISSN: | 1432-0614 online 0175-7598 print https://doi.org/10.1007/s00253-023-12856-y |
Appears in Collections: | 2414 Artículos |
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File | Description | Size | Format | |
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Long chain capsaicin analogues synthetized by CALB.pdf | Documento | 3.95 MB | Adobe PDF | View/Open |
Enlace a_Long chain capsaicin analogues synthetized by CALB.htm | Enlace a publicación | 47.45 kB | HTML | View/Open |
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